Journal: Biology Direct
Article Title: Partial inhibition of viral replication machinery enhances recombination in herpes simplex viruses
doi: 10.1186/s13062-025-00711-1
Figure Lengend Snippet: Viral polymerase inhibitors increase the rate of recombination between HSV1 and HSV2. A . schematic representation of HSV-1 expressing ICP4-YFP and VP26-RFP (ND2) and HSV-2 strain G and possible viral progeny (parental, recombinants) each with an example plaque. B . recombination rates in the presence of 2 µM acyclovir (purple) or 25 nM pritelivir (red) compared to control (gray) on HFF cells. Recombination rates were calculated by manually classifying dual-, single- and no-FP plaques. Data represent two independent experiments with two replicates each. Between 300 to 2735 plaques were classified for each replicate. Statistical significance was determined by ordinary one-way ANOVA (* < 0.05 ). Horizontal line represents the mean, and error bars indicate standard deviation. C . single-FP plaques from B were purified and analyzed from two independent experiments with two replicates each. Example PCR analysis for 2 purified recombinant single-FP plaques from either control (out of 19), acyclovir (out of 21) or pritelivir (out of 39) initial experiment. Expected specific amplicons for HSV-1 (green arrow) and HSV-2 (blue arrow) was ~135bp and ~94bp respectively. Example PCR for HSV-1 and HSV-2 was performed on lysed viral ND2 and OK200 stocks, respectively (note that HSV-1 US1 has a larger PCR fragment than expected). Dashed turquois line annotates the repeat genes; RL2 and RS1. D - E . For each plaque, the number of observed aberrations (crosses, deletions and duplications) detected by the PCR assay ( c ) was summed, D . frequency distribution of aberrations per plaque and E. the average number of aberrations per plaque, were plotted for each treatment. Colored as in B . statistical significance was determined by ordinary one-way ANOVA (* < 0.05 )
Article Snippet: HSV-2 strain G [VR-3393] was obtained from ATCC.
Techniques: Expressing, Control, Standard Deviation, Purification, Recombinant
ATCC is a verified supplier 
ATCC manufactures this product 
